PROTEOMICS
Top-down proteomics: Relatively few high abundance proteins (HAP), including albumin and immunoglobulins, comprise over 90% of the proteome by weight (see figure). In contrast, low abundance proteins (LAP) – including disease biomarkers – can be very difficult to identify and characterize due to the predominance of HAP. Therefore, removal of HAP prior to 2D-electrophoesis or LC/MS can significantly improve the odds of identifying novel biomarker proteins. ProteoMine™ is a novel, powerful, yet cost-effective approach to the removal of 97-99% HAP for proteomics research.
Bottom-up proteomics: An alternative approach to the identification of protein biomarkers involves selective isolation of protein subsets based on the premise that a specific subset is more likely to contain biomarker(s) of interest. Given the prominent role of kinases in the regulation of mammalian metabolism, several methods have been developed for selective isolation of phosphoprtoteins. Rather than focusing on kinase substrates, we offer an immunoaffinity method for the isolation of ATP-binding proteins, which includes kinases.
 
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