Human antiplasmin activity ELISA assay

Background

This human α-2-antiplasmin activity assay is for the quantitative determination of active α-2-antiplasmin in human plasma.
α-2-antiplasmin is the major circulating inhibitor of plasmin. It plays a role in the regulation of intravascular fibrinolysis (1,2). Decreased levels of α-2-antiplasmin may play an important role in the increased capacity of the fibrinolytic function and may be beneficial in the treatment of thrombotic diseases, acute pulmonary embolism, and hepatic repair (3,4,6,7).

Assay Principle

Functionally active α-2-antiplasmin present in plasma reacts with plasmin coated and dried on a microtiter plate. Latent or complexed a-2-antiplasmin will not bind to the plate or be detected. Unbound α-2-antiplasmin samples are aspirated and an anti-α-2-antiplasmin primary antibody is added. Excess primary antibody is then aspirated. The bound antibody, which is proportional to the original active α-2-antiplasmin present in the samples, is then reacted with a horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is used for color development at 450 nm. The amount of color development is directly proportional to the concentration of active α-2-antiplasmin in the sample.

Typical Standard Curve

References

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4.    Dewerchin, M., et al.: Thromb Haemost.; 86(2):640-6
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6.    Matsuno, H., et al.: (2003) J Thromb Haemost.; 1(8):1734-9
7.    Okada, K., et al.: (2004) J Hepatol.; 40(1):110-6
8.    Cederholm-Williams, S.A.: (1981) J Clin Pathol.; 34(9):979–981
9.    Takei, M., et al.: (2002) J Thromb Thrombolysis; 14(3):205-11